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KMID : 0604520140400030307
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Journal of the Society of Cosmetic Scientists of Korea
2014 Volume.40 No. 3 p.307 ~ p.311
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Study of 2,3,5-Triphenyltetrazolium Chloride for Detection of Pathogenic Microorganisms
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Kang Jung-Wook
Bae Joon-Tae
Yeon Jae-Young
Kim Young-Ho
Kim Jin-Hwa
Lee Geun-Soo
Pyo Hyeong-Bae
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Abstract
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2,3,5-Triphenyltetrazolium chloride (TTC) is used as a redox indicator in culture media. It is colorless in the oxidized form and is reduced to formazan, an insoluble pigment, by dehydrogenases in actively growing microbial cells. The aim of this study was to assess by microbial test of the pathogenic microorganisms using TTC reduction. The pathogenic microorganisms were reduced in medium by dehydrogenase to produce insoluble red formazan. We observed that the optimization method of TTC allowed more than 12 h incubation in 0.04% concentration. Also, the growth of microorganisms with media was increased formazan production. We confirmed that microorganisms were quickly observed to grow colonies cultured red color without affecting the growth of microorganisms. It is suggested that the microbial test using TTC can provide better and quicker test method in cosmetics development.
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KEYWORD
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2,3,5-triphenyltetrazolium chloride, microorganism, microbial test, 1,3,5, -triphenylformazan
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